Recombinant Human PABPN1 Protein

Beta LifeScience SKU/CAT #: BL-4073PS
Recombinant Human PABPN1 Protein

Recombinant Human PABPN1 Protein

Beta LifeScience SKU/CAT #: BL-4073PS
Catalog No.: BL-4073PS

Product Overview

Tag N/A
Host Species Human
Synonym Poly(A) Binding Protein Nuclear 1, Poly(A) Binding Protein 2, Polyadenylate-Binding Nuclear Protein 1, PABP-2, OPMD, PAB2, Nuclear Poly(A)-Binding Protein 1, PABII.
Background PABPN1 is an highly expressed nuclear protein which has high affinity to nascent poly(A) tails. PABPN1 is essential for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and regulates the length of the poly(A) tail to about 250 nt. In its steady-state, PABPN1 is restricted to the nucleus however there are different poly(A) binding proteins which can be found in the cytoplasm. PABPN1 has a GCG trinucleotide repeat at the 5' end of the coding region. Expansion of this repeat from the normal 6 copies to 8-13 copies results in autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Associated pseudogenes were located on chromosomes 19 and X. Additionally, there is a read-through transcription between PABPN1 gene and the adjacent upstream BCL2L2 (BCL2-like 2) gene.
Description PABPN1 Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 211a.a. (119-306) and having a molecular weight of 23.8 kDa. PABPN1 is fused to a 23a.a. His-tag at N-terminus.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSLEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSIEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGFAYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRAR YRARTTNYNS SRSRFYSGFN SRPRGRVYRG RARATSWYSP Y
Purity >95% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The PABPN1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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