Recombinant Human NSDHL Protein

Beta LifeScience SKU/CAT #: BL-4042PS
Recombinant Human NSDHL Protein

Recombinant Human NSDHL Protein

Beta LifeScience SKU/CAT #: BL-4042PS
Catalog No.: BL-4042PS

Product Overview

Tag N/A
Host Species Human
Synonym H105E3, SDR31E1, XAP104, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, Protein H105e3, NSDHL.
Background NAD (P) Dependent Steroid Dehydrogenase-Like (NSDHL) takes part in synthesis of cholesterol. NSDHL is involved in one of a few steps which convert lanosterol to cholesterol by removing a carbon atom and three hydrogen atoms (a methyl group). NSDHL is also a vital element of cell membranes and myelin, the fatty covering that insulates nerve cells.
Description NSDHL Human Recombinant expressed in E. coli is a single polypeptide chain containing 320a.a. (1-297) and having a molecular weight of 35.5kDa. NSDHL is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY.
Purity >80% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The NSDHL solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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