Recombinant Human NPHS2 Protein
Beta LifeScience
SKU/CAT #: BL-4037PS
Recombinant Human NPHS2 Protein
Beta LifeScience
SKU/CAT #: BL-4037PS
Catalog No.: BL-4037PS
Product Overview
| Tag | His |
| Host Species | Human |
| Synonym | Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin), PDCN, SRN1, Podocin, NPHS2. |
| Background | Nephrosis 2 Idiopathic Steroid-Resistant (NPHS2) is a protein which has a role in the regulation of glomerular permeability, serving as a linker between the plasma membrane and the cytoskeleton. NPHS2 gene mutations cause steroid-resistant nephrotic syndrome. |
| Description | NPHS2 Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain (Val125-Leu383) containing 269a.a. including a 10 a.a. His tag at N-terminus. The total calculated molecular weight is 30.2kDa. |
| Source | E.coli |
| AA Sequence | MKHHHHHHASVKVVQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLLNCLSSPS NRTQGSLPFP SPSKPVEPLN PKKKDSPML. |
| Purity | >95.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | NPHS2 was filtered (0.4µm) and lyophilized in 30mM acetate buffer and 1mM dithiothreitol, pH 4. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
