Recombinant Human NMNAT2 Protein

Name: Recombinant Human NMNAT2 Protein
Brand: Beta LifeScience
SKU: BL-2020PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Nicotinamide Nucleotide Adenylyltransferase 2, C1orf15, Nicotinate-NucleotideAdenylyltransferase 2, NaMN Adenylyltransferase 2, NMN Adenylyltransferase 2, PNAT2,KIAA0479, Chromosome 1 Open Reading Frame 15, Nicotinamide MononucleotideAdenylyltransferase 2, Pyridine Nucleotide Adenylyltransferase 2, EC 2.7.7.1, EC2.7.7.18.
Background	NicotinamideNucleotide Adenylyltransferase 2, also known as NMNAT2 is a member of thenicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, membersof which catalyze a vital step in NAD (NADP) biosynthetic pathway. Unlike theother human family member, which is localized to the nucleus, and isubiquitously expressed; NMNAT2 is cytoplasmic, and is predominantlyexpressed in the brain. Two transcript variants encoding different isoformshave been found for NMNAT2. Among the diseases associated with NMNAT2 aretauopathy, and systemic lupus erythematosus.
Description	NMNAT2 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 327a.a. (1-307 a.a) and having a molecular weight of 36.6kDa.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSHMTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMCQLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQNSNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIPGLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHGDGHVVDYLSQ PVIDYILKSQ LYINASG
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Bioactivity	Specific activity: > 500 pmol/min/ug. One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37°C.
Formulation	NMNAT2 protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.