Recombinant Human NFU1 Protein

Beta LifeScience SKU/CAT #: BL-4025PS
Recombinant Human NFU1 Protein

Recombinant Human NFU1 Protein

Beta LifeScience SKU/CAT #: BL-4025PS
Catalog No.: BL-4025PS

Product Overview

Tag N/A
Host Species Human
Synonym CGI-33, HIRIP, HIRIP5, MMDS1, Nfu, NifU, NIFUC, NFU1iron-sulfur cluster scaffold homolog, mitochondrial, HIRA-interacting protein 5.
Background NFU1 is a protein which is localized to mitochondria and plays a vital role in iron-sulfur cluster biogenesis. The NFU1 protein constructs and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. NFU1 gene mutations cause multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of the NFU1 gene are located on the short arms of chromosomes 1 and 3.
Description NFU1 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 268a.a. (10-254 a.a) and having a molecular weight of 29.9kDa.NFU1 is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPVRYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENEELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKGFEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP.
Purity >90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation NFU1 protein solution (0.5mg/ml) containing Phosphate buffer saline (pH 7.4), 20% glycerol and 1mM DTT.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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