Recombinant Human NFU1 Protein
Beta LifeScience
SKU/CAT #: BL-4025PS
Recombinant Human NFU1 Protein
Beta LifeScience
SKU/CAT #: BL-4025PS
Catalog No.: BL-4025PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | CGI-33, HIRIP, HIRIP5, MMDS1, Nfu, NifU, NIFUC, NFU1iron-sulfur cluster scaffold homolog, mitochondrial, HIRA-interacting protein 5. |
| Background | NFU1 is a protein which is localized to mitochondria and plays a vital role in iron-sulfur cluster biogenesis. The NFU1 protein constructs and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. NFU1 gene mutations cause multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of the NFU1 gene are located on the short arms of chromosomes 1 and 3. |
| Description | NFU1 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 268a.a. (10-254 a.a) and having a molecular weight of 29.9kDa.NFU1 is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPVRYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENEELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKGFEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP. |
| Purity | >90.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | NFU1 protein solution (0.5mg/ml) containing Phosphate buffer saline (pH 7.4), 20% glycerol and 1mM DTT. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
