Recombinant Human NANOG Protein (His Tag)

Beta LifeScience SKU/CAT #: BL-3569PS
Recombinant Human NANOG Protein (His Tag)

Recombinant Human NANOG Protein (His Tag)

Beta LifeScience SKU/CAT #: BL-3569PS
Catalog No.: BL-3569PS

Product Overview

Tag His
Host Species Human
Synonym NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Background NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
Description NANOG Human Recombinant expressed in E.coli is a single, non-glycosylated polypeptide chain containing 174a.a. (1-154) and having a molecular weight of 19.6 kDa.The NANOG is fused to a 20a.a. His-Tag at N-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ
Purity >90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation NANOG protein (0.25mg/ml) is supplied in 20mM Tris-HCL, pH-8, 1mM DTT and 20% Glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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