Recombinant Human MYL5 Protein
Beta LifeScience
SKU/CAT #: BL-3558PS
Recombinant Human MYL5 Protein
Beta LifeScience
SKU/CAT #: BL-3558PS
Catalog No.: BL-3558PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | Myosin light chain 5, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, MYLC2, MyLC-2, MYL5. |
| Background | Myosin regulatory light chain 5 (MYL5) is a hexameric ATPase cellular motor protein. Myosin is comprised of 2 heavy chains, 2 nonphosphorylatable alkali light chains, and 2 phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in the fetal muscle and in the adult retina, cerebellum, and basal ganglia. The reconstitution of myosin with MYL5 or alkali light chain increases filament velocity to intermediate rates, and the re-addition of both classes of light chains fully reinstates the original sliding pace. |
| Description | MYL5 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 156a.a. (1-132 a.a.) and having a molecular weight of 17.4kDa.MYL5 is fused to a 24a.a. His-tag at N-terminus and purified by unique purification methods. |
| Source | E.coli |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMDQNRD GFIDKEDLKD TYASLGKTNV KDDELDAMLK EASGPINFTM FLNLFGEKLS GTDAEETILN AFKMLDPDGK GKINKEYIKR LLMSQADKMT AEEVDQMFQF ASIDVAGNLD YKALSYVITH GEEKEE. |
| Purity | >90.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | MYL5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol and 0.1M NaCl. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
