Recombinant Human MTDH Protein

Beta LifeScience SKU/CAT #: BL-3995PS
Recombinant Human MTDH Protein

Recombinant Human MTDH Protein

Beta LifeScience SKU/CAT #: BL-3995PS
Catalog No.: BL-3995PS

Product Overview

Tag His
Host Species Human
Synonym Metadherin, Lysine-Rich CEACAM1 Co-Isolated Protein, Astrocyte Elevated Gene-1 Protein, Metastasis Adhesion Protein, Astrocyte Elevated Gene 1, 3D3/LYRIC, AEG-1, LYRIC, AEG1, LYRIC/3D3, 3D3, MTDH.
Background Metadherin (MTDH) downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. MTDH activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Furthermore, MTDH promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a central component in tumor cell expansion. In addition, MTDH promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Metadherin also induces chemoresistance.
Description Metadherin Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain (Val271-Asn456) containing 196a.a. including a 10 a.a. His tag at N-terminus. The total calculated molecular weight is 21.5kDa.
Source E.coli
AA Sequence MKHHHHHHASVSSGLNENLT VNGGGWNEKS VKLSSQISAG EEKWNSVSPA SAGKRKAEPS AWSQDTGDAN TNGKDWGRSW SDRSIFSGIG STAEPVSQST TSDYQWDVSR NQPYIDDEWS GLNGLSSADP NSDWNAPAEE WGNWVDEERA SLLKSQEPIP DDQKVSDDDK EKGEGALPTG KSKKKKKKKK KQGEDN.
Purity >90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation MTDH was filtered (0.4µm) and lyophilized in 20mM Tris buffer and 50mM NaCl, pH 7.5.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
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