Recombinant Human MTDH Protein
Beta LifeScience
SKU/CAT #: BL-3995PS
Recombinant Human MTDH Protein
Beta LifeScience
SKU/CAT #: BL-3995PS
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Synonym | Metadherin, Lysine-Rich CEACAM1 Co-Isolated Protein, Astrocyte Elevated Gene-1 Protein, Metastasis Adhesion Protein, Astrocyte Elevated Gene 1, 3D3/LYRIC, AEG-1, LYRIC, AEG1, LYRIC/3D3, 3D3, MTDH. |
Background | Metadherin (MTDH) downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. MTDH activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Furthermore, MTDH promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a central component in tumor cell expansion. In addition, MTDH promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Metadherin also induces chemoresistance. |
Description | Metadherin Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain (Val271-Asn456) containing 196a.a. including a 10 a.a. His tag at N-terminus. The total calculated molecular weight is 21.5kDa. |
Source | E.coli |
AA Sequence | MKHHHHHHASVSSGLNENLT VNGGGWNEKS VKLSSQISAG EEKWNSVSPA SAGKRKAEPS AWSQDTGDAN TNGKDWGRSW SDRSIFSGIG STAEPVSQST TSDYQWDVSR NQPYIDDEWS GLNGLSSADP NSDWNAPAEE WGNWVDEERA SLLKSQEPIP DDQKVSDDDK EKGEGALPTG KSKKKKKKKK KQGEDN. |
Purity | >90.0% as determined by SDS-PAGE. |
Endotoxin | <1.0 EU per μg by the LAL method. |
Formulation | MTDH was filtered (0.4µm) and lyophilized in 20mM Tris buffer and 50mM NaCl, pH 7.5. |
Stability | Recombinant protein is stable for 12 months at -70°C |
Usage | For Research Use Only |
Storage | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |