Recombinant Human MIS12 Protein
Beta LifeScience
SKU/CAT #: BL-3973PS
Recombinant Human MIS12 Protein
Beta LifeScience
SKU/CAT #: BL-3973PS
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Host Species | Human |
Synonym | hMis12, KNTC2AP, MTW1, Protein MIS12 homolog. |
Background | Protein MIS12 Belongs to the mis12 family. MIS12 is an element of the MIS12 complex, which is necessary for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex which comprised of MIS12,DSN1 , NSL1 and PMF-1. MIS12 is section of a network of complexes that supply microtubule attachment and generates pulling forces from depolymerization. |
Description | MIS12 Human Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 228a.a. (1-205 a.a.) and having a molecular weight of 26.5kDa. MIS12 is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods. |
Source | E.coli |
AA Sequence | MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS. |
Purity | >90.0% as determined by SDS-PAGE. |
Endotoxin | <1.0 EU per μg by the LAL method. |
Formulation | MIS12 protein solution (1mg/ml) containing 20mM Tris-HCl buffer, (pH 8.0) ,2M Urea and10% glycerol. |
Stability | Recombinant protein is stable for 12 months at -70°C |
Usage | For Research Use Only |
Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |