Recombinant Human MICB Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3363
Recombinant Human MICB Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3363
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&Fc |
Host Species | Human |
Accession | NP_005922.2 |
Synonym | PERB11.2 |
Background | MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+alphabeta T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases. |
Description | A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ala 23 |
AA Sequence | Met 1-Gly 298 |
Molecular Weight | The recombinant human MICB/Fc is a disulfide-linked homodimer. The reduced monomer consists of 524 a.a. and has a predicted molecular mass of 59.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB/Fc monomer is approximately 80-90 kDa due to glycosylation. |
Purity | >98% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Immobilized human His-NKG2D (78-216) at 10 ug/ml (100 ul/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |