Recombinant Human MED4 Protein

Beta LifeScience SKU/CAT #: BL-3451PS
Recombinant Human MED4 Protein

Recombinant Human MED4 Protein

Beta LifeScience SKU/CAT #: BL-3451PS
Catalog No.: BL-3451PS

Product Overview

Tag His
Host Species Human
Synonym Mediator of RNA polymerase II transcription subunit 4, Mediator complex subunit 4, Vitamin D3 receptor-interacting protein complex 36 kDa component, Activator-recruited cofactor 36 kDa component, TRAP/SMCC/PC2 subunit p36 subunit, DRIP36, ARC36, MED4, VDRIP, TRAP36, HSPC126, FLJ10956, RP11-90M2.2.
Background Mediator complex subunit 4 (MED4) is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED4 is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is able to activate nuclear receptors in a ligand-dependent manner. MED4 functions as a link to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. MED4 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Description MED4 expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 278a.a. (1-270 a.a.) and having a molecular weight of 30.7kDa.MED4 is fused to 8a.a. His Tag at C-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH.
Purity >80.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The MED4 protein solution contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 100mM NaCl.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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