Recombinant Human MAG Protein (Fc Tag)
Beta LifeScience SKU/CAT #: BLPSN-3274
Recombinant Human MAG Protein (Fc Tag)
Beta LifeScience SKU/CAT #: BLPSN-3274
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
|GMA, S-MAG, SIGLEC-4A, SIGLEC4A
|The myelin-associated glycoprotein (MAG) contains five immunoglobulin-like domains and belongs to the sialic-acid-binding subgroup of the Ig superfamily. MAG is a transmembrane glycoprotein of 1kDa localized in myelin sheaths of periaxonal Schwann cell and oligodendroglial membranes where it functions in glia-axon interactions. It appears to function both as a receptor for an axonal signal that promotes the differentiation, maintenance and survival of oligodendrocytes and as a ligand for an axonal receptor that is needed for the maintence of myelinated axons. MAG contains a carbohydrate epitope shared with other glycoconjugates that is a target antigen in autoimmune peripheral neuropathy associated with IgM gammopathy and has been implicated in a dying back oligodendrogliopathy in multiple sclerosis. MAG is considered as a transmembrane protein of both CNS and PNS myelin and it strongly inhibits neurite outgrowth in both developing cerebellar and adult dosal root ganglion neurons. In contrast, MAG promotes neurite outgrowth from newborn DRG neurons. Thus, MAG may be responsible for the lack of CNS nerve regeneration and may influce both temporally and spatially regeneration in the PNS.
|A DNA sequence encoding the human MAG (P20916-1) (Met1-Pro516) was expressed with the Fc region of human IgG1 at the C-terminus.
|Predicted N Terminal
|The recombinant human MAG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 738 a.a. and has a predicted molecular mass of 81.7 kDa. The apparent molecular mass of the protein is approximately 113 kDa in SDS-PAGE under reducing conditions.
|>95% as determined by SDS-PAGE
|< 1.0 EU per μg of the protein as determined by the LAL method
|Please contact us for detailed information
|Lyophilized from sterile PBS, pH 7.4.
|The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
|For Research Use Only
|Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth. Inhibits axon outgrowth by binding to RTN4R. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b.
|Cell membrane; Single-pass type I membrane protein. Membrane raft.
|Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
|Spastic paraplegia 75, autosomal recessive (SPG75)
|Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths. Detecte
Gene Functions References
- we observed two-way correlations between the MOG and MAG levels and the fractional anisotropy and mean diffusivity values in the white matter of the left middle frontal lobe, right inferior parietal lobe, and right supplementary motor area in major depressive disorder patients PMID: 29477585
- polyneuropathy associated with anti-MAG antibodies is less homogeneous. PMID: 26065001
- This study identify involvement of myelin-associated glycoprotein in this family with a disorder affecting the central and peripheral nervous system, and suggest that loss of the protein function is responsible for the unique clinical phenotype PMID: 26179919
- Results show that MAG is important for axon-glia contact in a model for Charcot-Marie-Tooth disease type 1A, and suggest that its increased expression in patients has a compensatory role in the disease pathology PMID: 22940629
- Increased serum levels of MAG (and MBP) were found in autistic patients with allergic manifestations compared to those without these manifestations. PMID: 23726766
- Primary mitochondrial respiratory chain defects affecting the white matter, and unrelated to inflammation, are associated with MAG loss and central nervous system demyelination. PMID: 22491194
- Distal acquired demyelinating symmetric neuropathy without anti-MAG antibodies is more likely to be considered a variant of chronic inflammatory demyelinating polyradiculoneuropathy, including a hematological or immunological condition. PMID: 21199182
- polysialylated NCAM persistence, up-regulated polysialyltransferase-1 mRNA and previously uncovered defective myelin-associated glycoprotein may be early pathogenetic events in adult-onset autosomal-dominant leukodystrophy PMID: 19725832
- Myelin destruction with preferential loss of MAG is found in autopsy brains with acute white matter ischemia as well as in HSV- and CMV-encephalitis. PMID: 12528815
- Possible association of MAG and schizophrenia in a Chinese Han cohort of family trios PMID: 15820319
- Our findings of a significant associations between schizophrenia and the MAG gene suggest that this gene may be involved in susceptibility to schizophrenia in the Chinese Han population. PMID: 16039057
- Expression of MAG, CNP and OLIG2 did not differ between patients with schizophrenia and controls in the grey or white matter PMID: 17964117
- These results support the hypothesis that the adhesive interactions between MUC1 and MAG are of biological significance in pancreatic cancer perineural invasion. PMID: 17974963
- Twenty of 46 patients with IgM amyloidosis (7 with and 13 without polyneuropathy) had elevation of anti-MAG or SGPG by enzyme-linked immunosorbent assay PMID: 18236455
- This finding provides support for potential association of the CNP gene but not the MAG gene in schizophrenia in a Caucasian population. PMID: 18496213
- In our patient, propriospinal myoclonus was associated with anti-MAG polyneuropathy, but the causal relationship remains unclear. PMID: 18816614
- RNF10 is a trans-acting protein regulating MAG expression and is required for myelin formation. PMID: 18941509
- Taken together, these findings suggest that in anti-MAG neuropathy patients, IgM deposits are entrapped within cutaneous perineurium-ensheathed nerve bundles where they accumulate in the endoneurial space. PMID: 19151627
- ELISA is more sensitive than Western blot to diagnose anti-myelin-associated glycoprotein related polyneuropathy, although a positive serology may be found in other forms of polyneuropathy as well. PMID: 19720975