Name: Recombinant Human Intrinsic Factor Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-3011
Availability: InStock

Recombinant Human Intrinsic Factor Protein (His Tag)

Catalog No.: BLPSN-3011

Product Overview

Tag	His
Host Species	Human
Accession	P27352
Synonym	IF, IFMH, INF, TCN3
Background	Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
Description	A DNA sequence encoding the human GIF (P27352-1) (Met1-Tyr417) was expressed with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Ser 19
AA Sequence	Met1-Tyr417
Molecular Weight	The recombinant human GIF consists of 410 a.a. and predicts a molecular mass of 44.8 KDa. It migrates as an approximately 49 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.