A DNA sequence encoding the human IgG3 Fc region (P01860) (Glu 99-Lys 377) was expressed and purified.
Predicted N Terminal
Glu 99-Lys 377
The recombinant human IgG3-Fc consists of 279 a.a. and has a predicted molecular mass of 31.2 kDa. As a result of glycosylation, the apparent molecular mass of IgG3-Fc is approximately 38 kDa in SDS-PAGE under reducing conditions.
>96% as determined by SDS-PAGE
< 1.0 EU per μg of the protein as determined by the LAL method
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Lyophilized from sterile PBS, pH 7.4.
The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
For Research Use Only
Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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