Recombinant Human HSP27 Protein

Beta LifeScience SKU/CAT #: BL-3284PS
Recombinant Human HSP27 Protein

Recombinant Human HSP27 Protein

Beta LifeScience SKU/CAT #: BL-3284PS
Catalog No.: BL-3284PS

Product Overview

Tag N/A
Host Species Human
Synonym HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Background HSP27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
Description Recombinant Human HSP-27 expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 205a.a. and having a molecular weight of 22.7 kDa. Hsp27 was over-expressed in E. coli and purified by conventional chromatography.
Source E.coli
AA Sequence MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK.
Purity >95.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation The HSP27 protein solution contains 20mM Hepes pH-7.5, 1mM DTT and 100mM KCl.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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