Recombinant Human HRG Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2456
Recombinant Human HRG Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2456
Catalog No.: BLPSN-2456
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_000403.1 |
| Synonym | HPRG, HRGP, THPH11 |
| Background | Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 35 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 1 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells. |
| Description | A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal His tag was expressed. |
| Source | HEK293 |
| Predicted N Terminal | Val 19 |
| AA Sequence | Met 1-Lys 525 |
| Molecular Weight | The secreted recombinant human HPRG comprises 518 a.a. with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. Human HPRG immobilized at 1 ug/ml (100 ul/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 ug/ml Concanvalin A. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
