Recombinant Human HMGN1 Protein
Beta LifeScience
SKU/CAT #: BL-3330PS
Recombinant Human HMGN1 Protein
Beta LifeScience
SKU/CAT #: BL-3330PS
Catalog No.: BL-3330PS
Product Overview
| Tag | N/A |
| Host Species | Human |
| Synonym | HMG14, GC104230, High-Mobility Group Nucleosome Binding Domain 1. |
| Background | HMGN1 binds to the inner side of the nucleosomal DNA therefore changing the interaction between the DNA and the histone octamer. HMGN1 participates in the development which maintains transcribable genes in an exceptional chromatin conformation. HMGN1 inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. |
| Description | HMGN1 Human Recombinant expressed in E.Coli is a single, non-glycosylated, polypeptide chain containing 108a.a. (1-100 a.a.) and having a molecular weight of 11.7 kDa. HMGN1 protein is fused to an 8a.a. His-Tag at C-terminus and purified by standard chromatography. |
| Source | E.coli |
| AA Sequence | MPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD LEHHHHHH. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | HMGN1 protein solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl, 0.1mM PMSF & 20% glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
