Name: Recombinant Human HLA-DRB1 Protein
Brand: Beta LifeScience
SKU: BL-3339PS
Availability: InStock

Catalog No.: BL-3339PS

Product Overview

Tag	His
Host Species	Human
Synonym	DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigen DRB1 16, DR-16, DR16, Human Leucocyte Antigen DRB1, MHC Class II HLA-DR-Beta Cell Surface Glycoprotein, MHC Class II HLA-DRw10-Beta.
Background	Major Histocompatibility Complex Class II DR Beta 1 also known as HLA-DRB1 ia a member of the HLA class II beta chain paralogs. Molecule class II is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. HLA-DRB1 takes an essential part in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Furthermore, the beta chain is approximately 26- 28 kDa. It is encoded by 6 exons. While exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Description	HLA-DRB1 expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 207a.a. (30-227a.a.) and having a molecular weight of 24.0kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). HLA-DRB1 is expressed with a 9a.a. His tag at C-Terminus and purified by unique purification methods.
Source	Sf9
AA Sequence	ADPGDTRPRF LWQPKRECHF FNGTERVRFL DRYFYNQEES VRFDSDVGEF RAVTELGRPD AEYWNSQKDI LEQARAAVDT YCRHNYGVVE SFTVQRRVQP KVTVYPSKTQ PLQHHNLLVC SVSGFYPGSI EVRWFLNGQE EKAGMVSTGL IQNGDWTFQT LVMLETVPRS GEVYTCQVEH PSVTSPLTVE WRARSESAQS KHHHHHH
Purity	>85.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	HLA-DRB1 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 30% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.