Recombinant Human HLA-DRB1 Protein

Beta LifeScience SKU/CAT #: BL-3338PS
Recombinant Human HLA-DRB1 Protein

Recombinant Human HLA-DRB1 Protein

Beta LifeScience SKU/CAT #: BL-3338PS
Catalog No.: BL-3338PS

Product Overview

Tag N/A
Host Species Human
Synonym DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, MHC class II antigenDRB1 16, DR-16, DR16, Human Leucocyte AntigenDRB1, MHC ClassIIHLA-DR-Beta Cell Surface Glycoprotein, MHC Class IIHLA-DRw10-Beta.
Background Major Histocompatibility Complex Class II DR Beta 1 also known as HLA-DRB1 ia a member of the HLA class II beta chain paralogs. Molecule class II is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. HLA-DRB1 takes an essential part in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Furthermore, the beta chain is approximately 26- 28 kDa. It is encoded by 6 exons. While exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.
Description HLA-DRB1 Human Recombinant expressed in E.Coli is asingle, non-glycosylated polypeptide chain containing 219a.a. (30-227 a.a)and having a molecular weight of 25.2kDa.HLA-DRB1 is fused to a 21a.a. His-tag atN-terminus and purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAEYWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEVRWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK
Purity Greaterthan 85% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation HLA-DRB1 protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
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