Name: Recombinant Human Histone H2B Protein
Brand: Beta LifeScience
SKU: BLPSN-2438
Availability: InStock

Catalog No.: BLPSN-2438

Product Overview

Tag	N/A
Host Species	Human
Accession	Q16778
Synonym	GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, MGC119802, MGC119804, MGC129733, MGC129734
Background	Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone proteins exhibit two levels of diversity: 1. evolutionary diversity between species and 2. subtype diversity in a class(H1, H2A, H2B, H3 or H4) within a species. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone H2B proteins have been studied in a variety of species and is easily detecred in most species. The reversible ubiquitylation of histone H2B has long been implicated in transcriptional activation and gene silencing. Phosphorylation of H2B serine 32 occurs in normal cycling and mitogen-stimulated cells. Notably, this phosphorylation is elevated in skin cancer cell lines and tissues compared with normal counterparts. HIST2H2BE is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Description	A DNA sequence encoding the human HIST2H2BE (Q16778) (Met 1-Lys 126) was expressed and purified.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met 1-Lys 126
Molecular Weight	The recombinant human HIST2H2BE consists of 126 a.a. and has a calculated molecular mass of 14.2 kDa. It migrates as an 16 kDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 2mM beta-Mercaptoethanol, pH 6.9.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.