Recombinant Human Her2 / ERBB2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2399

Recombinant Human Her2 / ERBB2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2399
Catalog No.: BLPSN-2399
Product Overview
Tag | Fc |
Host Species | Human |
Accession | NP_004439.2 |
Synonym | ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, erb-b2 receptor tyrosine kinase 2 |
Background | Receptor tyrosine-protein kinase erbB-2, also known as CD340 (cluster of differentiation 340), proto-oncogene Neu, Erbb2 (rodent), or ERBB2 (human), is a protein that in humans is encoded by the ERBB2 gene. ERBB is abbreviated from erythroblastic oncogene B, a gene isolated from avian genome. It is also frequently called HER2 (from human epidermal growth factor receptor 2) or HER2/neu. ERBB2, commonly referred to as HER2, is amplified and/or overexpressed in 20-30% of invasive breast carcinomas. HER2-positive breast cancer is treated in a separate manner from other subtypes of breast cancer and commonly presents as more aggressive disease. Metastatic HER2-positive breast cancer is now commonly treated with HER2-targeted therapy. Apart from being amplified/overexpressed, ERBB2 activating mutations have been shown to have clinical importance in HER2-negative breast cancer. These mutations have shown sensitivity to the tyrosine kinase inhibitor neratinib, and highlight the importance of clinical sequencing efforts in treating breast cancer. |
Description | A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Thr 23 |
AA Sequence | Met 1-Thr 652 |
Molecular Weight | The recombinant human ErbB2/Fc chimera is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The monomer comprises 868 amino acids and has a calculated molecular mass of 96.1 kDa. As a result of glycosylation, the monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 ug/ml can bind Herceptin with a linear range of 3.2-80 ng/ml. |
Formulation | Recombinant Human Her2 / ERBB2 Protein (Fc Tag) was lyophilized from sterile PBS, pH 7.4 |
Stability | Recombinant Human Her2 / ERBB2 protein is stable up to 1 year at -70°C |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |