Recombinant Human H2AFZ Protein

Beta LifeScience SKU/CAT #: BL-3867PS
Recombinant Human H2AFZ Protein

Recombinant Human H2AFZ Protein

Beta LifeScience SKU/CAT #: BL-3867PS
Catalog No.: BL-3867PS

Product Overview

Tag N/A
Host Species Human
Synonym H2A Histone Family, Member Z, H2AZ, H2A/z, H2A.Z-1, H2A.z,H2AZ Histone, Histone H2A.Z.
Background HistoneH2A.Z (H2AFZ) belongs to the histone H2A family. Histones are basic nuclearproteins which are responsible for the nucleosome structure of the chromosomalfiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrappedaround a histone octamer composed of pairs of each of the four core histones(H2A, H2B, H3, and H4). H2AFZ is implicated in the formation of constitutiveheterochromatin and vital for chromosome segregation during cell division.Moreover, variant Histone H2A, which takes the place of conventional H2A in asubset of nucleosomes. Studies in mice have demonstrated that this particularhistone is essential for embryonic development and show that lack of functionalhistone H2A leads to embryonic lethality.
Description H2AFZ Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 151a.a. (1-128 a.a) andhaving a molecular weight of 15.9kDa.H2AFZ is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMAGGKAG KDSGKAKTKA VSRSQRAGLQ FPVGRIHRHL KSRTTSHGRVGATAAVYSAA ILEYLTAEVL ELAGNASKDL KVKRITPRHL QLAIRGDEEL DSLIKATIAG GGVIPHIHKSLIGKKGQQKT V
Purity Greaterthan 85% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation H2AFZ protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
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