Recombinant Human H2AFZ Protein

Name: Recombinant Human H2AFZ Protein
Brand: Beta LifeScience
SKU: BL-3867PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	H2A Histone Family, Member Z, H2AZ, H2A/z, H2A.Z-1, H2A.z,H2AZ Histone, Histone H2A.Z.
Background	HistoneH2A.Z (H2AFZ) belongs to the histone H2A family. Histones are basic nuclearproteins which are responsible for the nucleosome structure of the chromosomalfiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrappedaround a histone octamer composed of pairs of each of the four core histones(H2A, H2B, H3, and H4). H2AFZ is implicated in the formation of constitutiveheterochromatin and vital for chromosome segregation during cell division.Moreover, variant Histone H2A, which takes the place of conventional H2A in asubset of nucleosomes. Studies in mice have demonstrated that this particularhistone is essential for embryonic development and show that lack of functionalhistone H2A leads to embryonic lethality.
Description	H2AFZ Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 151a.a. (1-128 a.a) andhaving a molecular weight of 15.9kDa.H2AFZ is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMAGGKAG KDSGKAKTKA VSRSQRAGLQ FPVGRIHRHL KSRTTSHGRVGATAAVYSAA ILEYLTAEVL ELAGNASKDL KVKRITPRHL QLAIRGDEEL DSLIKATIAG GGVIPHIHKSLIGKKGQQKT V
Purity	Greaterthan 85% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	H2AFZ protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.