Recombinant Human GRK5 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2331
Recombinant Human GRK5 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2331
Catalog No.: BLPSN-2331
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_005299.1 |
| Synonym | GPRK5 |
| Background | G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease. |
| Description | A DNA sequence encoding the human GRK5 (NP_005299.1) (Met 1-Ser 590) was fused with a His tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Ser 590 |
| Molecular Weight | The recombinant human GRK5 consists of 600 a.a. and predicts a molecular mass of 69 kDa. The apparent molecular mass of rhGRK5 is approximately 58 kDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 1mM DTT, 0.5mM EDTA, 10% gly, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
