Recombinant Human GPHA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2310
Recombinant Human GPHA2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2310
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q96T91 |
| Synonym | A2, GPA2, ZSIG51 |
| Background | GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activates human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named as thyrostimulin based on its thyroid-stimulating activity. The expression of thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism. |
| Description | A DNA sequence encoding the human GPHA2 (Q96T91) (Met1-Tyr129) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 24 |
| AA Sequence | Met1-Tyr129 |
| Molecular Weight | The recombinant human GPHA2 consists of 117 a.a. and predicts a molecular mass of 13.1 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
