Recombinant Human Glyoxalase II Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2272

Recombinant Human Glyoxalase II Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2272
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag His
Host Species Human
Accession Q16775
Synonym GLO2, GLX2, GLXII, HAGH1
Background The human and rodent forms of glyoxalase II (Hydroxyacylglutathione hydrolase, HAGH) can readily be separated by starch gel electrophoretic procedures.
Description A DNA sequence encoding the mature form of human HAGH isoform 2 (Q16775-2) (Met 1-Asp 260) was fused with a His tag at the C-terminus
Source E.coli
Predicted N Terminal Met
AA Sequence Met 1-Asp 260
Molecular Weight The recombinant human HAGH consisting of 270 a.a. and has a calculated molecular mass of 30.2 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions
Purity >96% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 20mM Tris, 0.15 M NaCl, 10% glycerol, pH 7.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Subcellular Location [Isoform 1]: Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
Protein Families Metallo-beta-lactamase superfamily, Glyoxalase II family
Database References
Tissue Specificity Expressed in liver and kidney.

Gene Functions References

  1. Down regulation of Glyoxalase II was observed in cases of diabetic retinopathy as compared to controls. PMID: 29950256
  2. The critical role of glyoxalases as regulators of tumorigenesis in the prostate through modulation of various critical signaling pathways, and an overview of the current knowledge on glyoxalases in bladder, kidney and testis cancers is reviewed. (GLO1, GLO2) PMID: 29385039
  3. epidermal expression stronger in older skin donors PMID: 26914966
  4. Glo2, together with Glo1, represents a novel mechanism in prostate cancer progression as part of a pathway driven by PTEN/PI3K/AKT/mTOR signaling. PMID: 27696457
  5. This article reports for the first time a possible additional role of Glo2 that, after interacting with a target protein, is able to promote S-glutathionylation. PMID: 27935136
  6. This study suggested that HAGH, rs11859266 and rs3743852 showed significant associations with schizophrenia in males at allelic and genotype levels. PMID: 25645869
  7. No association between genetic variants of the HAGH gene and autism spectrum disorder was found. PMID: 24671236
  8. hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II PMID: 15117945
  9. Overexpression of glyoxalase II is associated with kidney tumor PMID: 16803681
  10. Data show that the GLX2 gene, which encodes glyoxalase II enzyme, is up-regulated by p63 and p73. PMID: 16831876
  11. In androgen-dependent prostate cancer cells, testosterone upregulates GLO2 mRNA levels. In androgen-independent prostate cancer cells, it downregulates GLO2 mRNA. PMID: 18344682
  12. Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme PMID: 19413286


Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

Recently viewed