Recombinant Human GGCT Protein (His Tag)

Name: Recombinant Human GGCT Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2244
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	O75223
Synonym	C7orf24, CRF21, GCTG, GGC
Background	GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Description	A DNA sequence encoding the mature form of human GGCT (O75223-1) (Met1-Leu188) was expressed with a His tag at the N-terminus.
Source	E.coli
Predicted N Terminal	His
AA Sequence	Met1-Leu188
Molecular Weight	The recombinant human GGCT consists of 203 a.a. and predicts a molecular mass of 22.8 KDa. It migrates as an approximately 23-26 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides and may play a significant role in glutathione homeostasis. Induces release of cytochrome c from mitochondria with resultant induction of apoptosis.
Protein Families	Gamma-glutamylcyclotransferase family
Database References	HGNC: 21705 OMIM: 137170 KEGG: hsa:79017 STRING: 9606.ENSP00000275428 UniGene: Hs.530024

Gene Functions References

GGCT is highly upregulated in HGSC tissues and associated with FIGO stage, lymph node metastasis and ascitic fluid volume. High expression of GGCT is associated with poor survival in HGSC patients. PMID: 29429592
Taken together, the results indicate that PHB2 plays a central role in p21 upregulation following GGCT knockdown and as such may promote deregulated proliferation of cancer cells by suppressing p21. PMID: 29307834
GGCT plays a critical role in glioma cell proliferation and may be a potential cancer therapeutic target. PMID: 26828272
GGCT is promising as a diagnostic marker and a therapeutic target for various cancers. This review summarizes these interesting findings. PMID: 26339607
G6PD, GGCT, IDH1, isocitrate dehydrogenase 2 (NADP+,mitochondrial) (IDH2) and glutathione S-transferase pi 1(GSTP1), five of the critical components of GSH pathway, contribute to chemoresistance. PMID: 25818003
GGCT plays a critical role in lung cancer cell proliferation. PMID: 25941902
Transcriptional silencing of the C7orf24 gene in the non-malignant cells is elicited through heterochromatin formation in its promoter region. PMID: 23853312
increased expression of GGCT was a common event among invasive esophageal squamous cell carcinoma tissues regardless of the depth of invasion. Lymph node metastasis and tumor differentiation correlated with GGCT expression PMID: 24342434
C7orf24 has been recognized as a potent marker for bladder and breast cancers. Studies on the role of gamma-glutamylcyclotransferace may aid in the control of malignant transformation of these cancers. PMID: 23513927
GGCT may be a biomarker of tumors in a limited range of organs. PMID: 22205682
C7orf24 overexpression defines a subgroup of breast tumors with poor clinical outcome. PMID: 20527979
CRF21 might play an important role in the induction of apoptosis by GGO in leukemia U937 cells. PMID: 16765912
presence of extra electron density/ligands and conservation at the sequence and structure levels, it is likely that the cavity has a crucial role in the function of LOC79017 and its structural neighbors possibly as an active site or ligand-binding site PMID: 17932939
The GGCT gene is located on chromosome 7p14-15 and consists of four exons that span 8 kb, the primary sequence is 188 amino acids in length and is unlike any protein of known function PMID: 18515354

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.