Recombinant Human GFR alpha-3 / GDNFR3 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2240
Recombinant Human GFR alpha-3 / GDNFR3 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2240
Catalog No.: BLPSN-2240
Product Overview
| Tag | His&Fc |
| Host Species | Human |
| Accession | NP_001487.2 |
| Synonym | GDNFR3 |
| Background | Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor alpha3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons. |
| Description | A DNA sequence encoding the human GFRa3 (NP_001487.2) (Met 1-Trp 382) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Asp 32 |
| AA Sequence | Met 1-Trp 382 |
| Molecular Weight | The recombinant human GFRa3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 599 a.a. and predicts a molecular mass of 67.3 kDa. As a result of glycosylation, the rh GFRa3/Fc monomer migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >80% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to bind mouse ARTN in a functional ELISA. |
| Formulation | Lyophilized from sterile PBS, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
