Recombinant Human GFPT1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2232
Recombinant Human GFPT1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2232
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Host Species | Human |
Accession | AAA58502.1 |
Synonym | CMSTA1, GFA, GFAT, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG |
Background | Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system. |
Description | The sequence corresponding to amino acids (Gln 332-Glu 699) of human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | AAA58502.1 |
Molecular Weight | The recombinant human GFAT exists as a homotetramer protein. The GFAT monomer consists of 370 a.a. and predicts a molecular mass of 41.5 kDa as estimated in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4, 10% glycerol. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |