Name: Recombinant Human Germinal Center Kinase / GCK Protein (His & GST Tag)
Brand: Beta LifeScience
SKU: BLPSN-2228
Availability: InStock

Recombinant Human Germinal Center Kinase / GCK Protein (His & GST Tag)

Catalog No.: BLPSN-2228

Product Overview

Tag	His&GST
Host Species	Human
Accession	AAH47865.1
Synonym	BL44, GCK, MAP4K2, RAB8IP
Background	Mitogen-activated protein kinase kinase kinase kinase 2, also known as B lymphocyte serine/threonine-protein kinase, Germinal center kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, Rab8-interacting protein and MAP4K2, is a cytoplasm and peripheral membrane protein which belongs to theprotein kinase superfamily, STE Ser/Thr protein kinase family and STE2 subfamily. MAP4K2 contains oneCNH domain and oneprotein kinase domain. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. MAP4K2 can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. It is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1 / MEKK1. MAP4K2 enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It may also play a role in the regulation of vesicle targeting or fusion.
Description	A DNA sequence encoding the human MAP4K2 (AAH47865.1) (Met 1-Tyr 812) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Met
AA Sequence	Met 1-Tyr 812
Molecular Weight	The recombinant human MAP4K2/GST chimera consists of 1049 a.a. and has a calculated molecular mass of 119 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
Purity	>87% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.