Recombinant Human GBA3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2207
Recombinant Human GBA3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2207
Catalog No.: BLPSN-2207
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_066024.1 |
| Synonym | CBG, CBGL1, GBA3, GLUC, KLrP, MGC104276, MGC126878 |
| Background | Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide. |
| Description | A DNA sequence encoding the human GBA3 (NP_066024.1) (Met 1-Leu 469) was fused with a His tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met 1 |
| AA Sequence | Met 1-Leu 469 |
| Molecular Weight | The recombinant human GBA3 consists of 480 a.a. and predicts a molecular mass of 55 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to hydrolyze 4-methylumbelliferyl-beta-D glucopyranoside. The specific activity is >1,500 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
