Recombinant Human Gastrokine 1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2205

Recombinant Human Gastrokine 1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-2205
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Product Overview

Tag His
Host Species Human
Accession Q9NS71
Synonym AMP18, BRICD1, CA11, FOV, foveolin, UNQ489/PRO1005
Background Gastrokine 1 (GKN1) belongs to the BRICHOS domain family and plays a major role in maintaining gastric mucosa integrity. GKN1 is highly expressed in gastric tissue and is secreted into the stomach but is not expressed in gastric cancer. GKN1-mediated inhibition of APP processing might represent a new approach for the prevention and therapy of Alzheimer's disease (AD). In the presence of GKN2, GKN1 loses its ability to decrease cell proliferation, induce apoptosis, and inhibit epigenetic alterations in gastric cancer cells, that GKN2 may contribute to the homeostasis of gastric epithelial cells by inhibiting GKN1 activity. The loss of GKN1 function contributes to malignant transformation and proliferation of gastric epithelial cells in gastric carcinogenesis.
Description A DNA sequence encoding the human GKN1 (Q9NS71) (Asn35-Asn199) was expressed with a C-terminal His tag.
Source Baculovirus-Insect Cells
Predicted N Terminal Asn 35
AA Sequence Asn35-Asn199
Molecular Weight The secreted recombinant human GKN1 consists of 175 a.a. and predicts a molecular mass of 19.6 KDa. The apparent molecular mass of the protein is approximately 24 KDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium.
Subcellular Location Secreted. Cytoplasmic granule. Golgi apparatus.
Protein Families Gastrokine family
Database References
Tissue Specificity Expressed in stomach (at protein level). No expression is detected in cancer tissue or gastric cancer cell lines.

Gene Functions References

  1. Data show that ubiquitin protein ligase E3 component n-recognin 5 protein (UBR5) bound the tumor suppressor gastrokine 1 (GKN1) and increased its ubiquitination to reduce the protein stability of GKN1. PMID: 27590582
  2. These results did not confirm GKN1 as a potential biomarker for gastric cancer. PMID: 27452910
  3. Low GKN1 expression is associated with gastric cancer. PMID: 27250838
  4. GKN1 may contribute to the maintenance of gastric epithelial homeostasis and inhibit gastric carcinogenesis by down-regulating the gastrin-cholecystokinin B receptor signaling pathway. PMID: 25752269
  5. Gastrokine 1 indirectly participates in actin stabilization since its overexpression in gastric cancer cells strongly increases the expression of tight and adherens junction proteins PMID: 26008777
  6. AMP-18 appears to act through PI3K/AKT pathways to increase p21 phosphorylation, thereby reducing its nuclear accumulation to overcome the antiproliferative effects of TNF-alpha. PMID: 25919700
  7. Results suggest a potential association between CRP and GPC5 variants with lung cancer risk; variation in GKN1 is associated with chemotherapy response in the Chinese Han population. PMID: 25999661
  8. GKN1 inhibits metastasis in gastric cancer cells. PMID: 25725220
  9. GKN1 may shorten telomeres by acting as a potential c-myc inhibitor that eventually leads to senescence and apoptosis in gastric cancer cells PMID: 25344918
  10. GKN1 expression in the gastric mucosa of gastric cancer patients is decreased; this is associated with progression and prognosis of gastric cancer. PMID: 25469040
  11. Data show that recombinant gastrokine 1 (GKN1) interacts with amyloid-beta peptide (1-40) preventing its aggregation. PMID: 25139219
  12. These results suggest that GKN1 might be a target to inhibit the effects from H.pylori CagA. PMID: 25239641
  13. Low GKN1 activity is associated with gastric cancers. PMID: 23812904
  14. Epstein-Barr virus EBNA1 binds to the divergent promoter of the GKN1 and GKN2 genes and contributes to the complex transcriptional and epigenetic deregulation of the GKN1 and GKN2 tumor suppressor genes. PMID: 24460791
  15. Our results show that GKN1 has an miR-185-dependent and -independent mechanism for chromatic and DNA epigenetic modification, thereby regulating the cell cycle PMID: 23846337
  16. Data suggest GKN1 is expressed in placental trophoblasts in third trimester (not first); GKN1 expression is limited to extravillous trophoblasts. GKN1 expression is downregulated in choriocarcinoma; GKN1 attenuates migration of choriocarcinoma cells. PMID: 23993393
  17. These data demonstrate that in the presence of GKN2, GKN1 loses its ability to decrease cell proliferation, induce apoptosis, and inhibit epigenetic alterations in gastric cancer cells. PMID: 24151046
  18. these results suggest that the NH2-terminal hydrophobic region and BRICHOS domain of GKN1 are sufficient for its tumor suppressor activities. PMID: 24099765
  19. the GKN1 gene may inhibit progression of gastric epithelial cells to cancer cells by regulating NF-kappaB signaling pathway and cytokine expression. PMID: 23444260
  20. Molecular and functional properties of human GKN1 indicate a more significant propensity for beta-sheet structures. PMID: 23319233
  21. Low expression of gastrokine 1 is associated with gastric cancer. PMID: 23317277
  22. Expression of GKN1 is progressively lost from normal mucosa, precancerous to cancerous gastric tissues, while restoration of GKN1 expression induces gastric cancer cells to undergo apoptosis. PMID: 22621392
  23. There is no direct molecular interaction between GKN1 and TFF1 in protein level in gastric cancer. PMID: 22842654
  24. Both Helicobacter pylori infection and long-term non-steroidal anti-inflammatory drug administration downregulate gastrokine-1 expression in the gastric mucosa, which may contribute to the gastric mucosal injuries induced by these two factors. PMID: 22798109
  25. GKN1 inhibits gastric cancer cell proliferation. PMID: 21751384
  26. The GKN1 gene may play an important role in the progression of sporadic gastric cancers via inhibition of EMT and cancer cell migration PMID: 21898090
  27. GKN1 modulates apoptotic signals and might play an important role for tissue repair during the early stages of neoplastic transformation. PMID: 21792914
  28. AMP-18 could protect the intestinal mucosal barrier by acting on specific tight junction proteins and stabilizing perijunctional actin PMID: 15961882
  29. study demonstrated the presence of gastrokine-1 isoforms of which the basic isoform was reduced in a subset of patients with H. pylori infection. PMID: 17092786
  30. GKN1 and GKN2 expression occurs frequently in gastric adenocarcinomas, especially in the diffuse subtype PMID: 18593995
  31. GKN1 expression is related to the inflammatory damage of gastric mucosa and could be the related to the gastric cellular phenotype. PMID: 18927498


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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