Recombinant Human FHL3 Protein

Name: Recombinant Human FHL3 Protein
Brand: Beta LifeScience
SKU: BL-3206PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Synonym	Four And A Half LIM Domains 3, SLIM2, Skeletal MuscleLIM-Protein 2, FHL-3, SLIM-2, Four And A Half LIM Domains Protein 3, LIM-OnlyProtein FHL3.
Background	Fourand a half LIM domains 3 (FHL3) belongs to a family of proteins containing afour-and-a-half LIM domain, which is a highly conserved double zinc fingermotif. FHL3 has been shown to interact with the cancer developmental regulatorsSMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and thehigh-affinity IgE beta chain regulator MZF-1. FHL3 is involved in tumorsuppression, repression of MyoD expression, and repression of IgE receptorexpression. Two transcript variants encoding different isoforms have been foundfor this gene.
Description	FHL3 Human Recombinant expressed in E.Coli is a single,non-glycosylated polypeptide chain containing 303a.a. (1-280 a.a) andhaving a molecular weight of 33.6kDa.FHL3 is fused to a 23a.a. His-tag at N-terminusand purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMSESFDC AKCNESLYGR KYIQTDSGPY CVPCYDNTFA NTCAECQQLI GHDSRELFYE DRHFHEGCFR CCRCQRSLAD EPFTCQDSEL LCNDCYCSAF SSQCSACGET VMPGSRKLEY GGQTWHEHCF LCSGCEQPLG SRSFVPDKGA HYCVPCYENK FAPRCARCSK TLTQGGVTYR DQPWHRECLV CTGCQTPLAG QQFTSRDEDP YCVACFGELF APKCSSCKRP IVGLGGGKYV SFEDRHWHHN CFSCARCSTS LVGQGFVPDG DQVLCQGCSQ AGP.
Purity	Greaterthan 90.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	FHL3 protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.