Recombinant Human FGFR2 Protein (His&Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2065
Recombinant Human FGFR2 Protein (His&Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2065
Catalog No.: BLPSN-2065
Product Overview
| Tag | His&Fc |
| Host Species | Human |
| Accession | NP_000132.3 |
| Synonym | FGFR2, BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, fibroblast growth factor receptor 2 |
| Background | Fibroblast growth factor receptor 2 (FGFR2) also known as CD332 (cluster of differentiation 332) is a protein that in humans is encoded by the FGFR2 gene residing on chromosome 10. FGFR2 is a receptor for fibroblast growth factor.FGFR2 has important roles in embryonic development and tissue repair, especially bone and blood vessels. Like the other members of the fibroblast growth factor receptor family, these receptors signal by binding to their ligand and dimerisation (pairing of receptors), which causes the tyrosine kinase domains to initiate a cascade of intracellular signals. FGFR2 has two naturally occurring isoforms, FGFR2IIIb and FGFR2IIIc, created by splicing of the third immunoglobulin-like domain. FGFR2IIIb is predominantly found in ectoderm derived tissues and endothelial organ lining, i.e. skin and internal organs |
| Description | A DNA sequence encoding the human FGFR2 extracellular domain (Met 1-Glu 377) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Arg 22 |
| AA Sequence | Met 1-Glu 377 |
| Molecular Weight | The recombinant human FGFR2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 604 amino acids and has a predicted molecular mass of 67.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFGFR2/Fc monomer is approximately 110-120 kDa due to glycosylation. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to inhibit FGF-acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts.The ED50 for this effect is typically 0.5-2.5 ng/ml. |
| Formulation | Recombinant Human FGFR2 Protein (His&Fc Tag) was lyophilized from sterile PBS, pH 7.4 |
| Stability | Recombinant Human FGFR2 protein is stable up to 1 year at -70°C |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
