Recombinant Human FES Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-2024
Recombinant Human FES Protein (His & GST Tag)

Recombinant Human FES Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-2024
Catalog No.: BLPSN-2024

Product Overview

Tag His&GST
Host Species Human
Accession P07332
Synonym FPS
Background Proto-oncogene tyrosine-protein kinase Fes/Fps, also known as Proto-oncogene c-Fes, Proto-oncogene c-Fps, Feline sarcoma oncogene, FES and FPS, is a protein which contains oneFCH domain, oneprotein kinase domain and oneSH2 domain. FES is a non-receptor protein tyrosine kinase expressed in hematopoietic progenitors and differentiated myeloid cells. FES is observed in the nuclear, granular and plasma membrane fractions of primary human neutrophils and the myeloid leukemia cell line, HL-6. The nuclear localization is confirmed by immunocytochemistry of neutrophils. FES has been implicated in granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and erythropoietin signal transduction. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. FES is also involved in normal hematopoiesis. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia.
Description A DNA sequence encoding the human FES (P07332-1) (Met 1-Arg 822) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Met 1-Arg 822
Molecular Weight The recombinant human FES/GST chimera consists of 1060 a.a. and has a calculated molecular mass of 121 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
Purity >75% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The specific activity was determined to be 200 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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