Recombinant Human FAM3B Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1977
Recombinant Human FAM3B Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1977
Catalog No.: BLPSN-1977
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | P58499 |
| Synonym | 42056, C21orf11, C21orf76, ORF9, PANDER, PRED44 |
| Background | Pancreatic derived factor, also known as FAM3B, is an islet-specific secreted cytokine specifically expressed at high levels in the islets of Langerhans of the endocrine pancreas. FAM3B protein is present in alpha- and beta- cells of pancreatic islets, insulin-secreting beta-TC3 cells, and glucagon-secreting alpha-TC cells. FAM3B causes apoptosis of beta-cells as assessed by electron microscopy, annexin -…¤ fluorescent staining, and flow-cytometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. FAM3B activated caspase-3 while not affect cytosolic Ca2+ levels or nitric oxide levels. Hense, FAM3B may have a role in the process of pancreatic?-cell apoptosis of primary islet and cell lines. FAM3B secretion is regulated by glucose and other insulin secretagogues. This islet-specific secreted cytokine is secreted from both pancreatic alpha- and beta- cells. Glucose stimulates FAM3B secretion dose dependently in beta- cell lines and primary islets but not in alpha-cells. It is likely cosecreted with insulin via the same regulatory mechanisms and structure and conformation is vital for FAM3B secretion. |
| Description | A DNA sequence encoding the human FAM3B isoform B (P58499-1) (Met 1-Ser 235) was fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 30 |
| AA Sequence | Met 1-Ser 235 |
| Molecular Weight | The secreted recombinant human FAM3B/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 447 a.a. and has a predicted molecular mass of 50 kDa. The apparent molecular mass of rhFAM3B/Fc monomer is approximately 57 kDa in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
