Name: Recombinant Human Factor XIII Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1969
Availability: InStock

Recombinant Human Factor XIII Protein (His Tag)

Catalog No.: BLPSN-1969

Product Overview

Tag	His
Host Species	Human
Accession	P05160
Synonym	Coagulation factor 13, Coagulation factor XIII, FXIIIB
Background	Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 1 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Description	A DNA sequence encoding the human F13B (P05160) (Met 1-Thr 661) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Glu 21
AA Sequence	Met 1-Thr 661
Molecular Weight	The secreted recombinant human F13B consists of 652 a.a. and predictes a molecular mass of 74.5 kDa. In SDS-PAGE under non-reduced conditions, the apparent molecular mass of rh F13B is approximately 65 kDa.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.