Recombinant Human Factor XIII Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1969
Recombinant Human Factor XIII Protein (His Tag)

Recombinant Human Factor XIII Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1969
Catalog No.: BLPSN-1969

Product Overview

Tag His
Host Species Human
Accession P05160
Synonym Coagulation factor 13, Coagulation factor XIII, FXIIIB
Background Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 1 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Description A DNA sequence encoding the human F13B (P05160) (Met 1-Thr 661) was expressed, fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Glu 21
AA Sequence Met 1-Thr 661
Molecular Weight The secreted recombinant human F13B consists of 652 a.a. and predictes a molecular mass of 74.5 kDa. In SDS-PAGE under non-reduced conditions, the apparent molecular mass of rh F13B is approximately 65 kDa.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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