Recombinant Human ErbB1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BL-0230NP
Recombinant Human ErbB1 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BL-0230NP
Catalog No.: BL-0230NP
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | P00533 |
| Synonym | ErbB1 Epidermal growth factor receptor; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR |
| Background | The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target. |
| Description | Recombinant Human Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1 is expressed by our Mammalian expression system and the target gene encoding Leu25-Val30Gly&Asn298-Ser645 is expressed with a Fc tag at the C-terminus. |
| Source | Human Cells |
| AA Sequence | Leu25-Val30Gly&Asn298-Ser645 |
| Purity | >95% as determined by reducing SDS-PAGE. |
| Endotoxin | <0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Recombinant Human ErbB1 Protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Dissolve the lyophilized protein in distilled water. |
| Usage | For Research Use Only |
| Storage | Human ErbB1 protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
