Recombinant Human ERAP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1904
Recombinant Human ERAP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1904
Catalog No.: BLPSN-1904
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_071745.1 |
| Synonym | FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP, LRAP.ERAP2 |
| Background | Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 96-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. |
| Description | A DNA sequence encoding the lumenal domain of human ERAP2 (NP_071745.1) (Ala 56-Thr 960) was expressed with a His tag at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Leu 24 |
| AA Sequence | Ala 56-Thr 960 |
| Molecular Weight | The recombinant human ERAP2 consists of 921 a.a. and predictes a molecular mass of 106 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 115-125 kDa due to glycosylation. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is >50 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 12.5mM Tris, 75mM NaCl, pH 7.5, 50% glycercol. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
