Recombinant Human Ephrin-B1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1890
Recombinant Human Ephrin-B1 Protein (His Tag)

Recombinant Human Ephrin-B1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1890
Catalog No.: BLPSN-1890

Product Overview

Tag His
Host Species Human
Accession NP_004420.1
Synonym CFND, CFNS, EFB1, EFL3, Elk-L, EPLG2, LERK2
Background Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.
Description A DNA sequence encoding the human EFNB1 (NP_004420.1) extracellular domain (Met 1-Gly 232) was fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Leu 28
AA Sequence Met 1-Gly 232
Molecular Weight The recombinant human EFNB1 consists of 221 a.a. and predicts a molecular mass of 24.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB1 is approximately 38 kDa due to glycosylation.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant human EphrinB1 at 10 ug/ml (100 ul/well) can bind human EphB6 with a linear range of 0.16-4 ug/ml.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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