Recombinant Human Ephrin A4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1859
Recombinant Human Ephrin A4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1859
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | NP_005218.1 |
| Synonym | EFL4, EPLG4, LERK4 |
| Background | EPH-related receptor tyrosine kinase ligand 4 (Ephrin-A4) also known as EFNA4, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A4/EFNA4 functions as a cell surface GPI-bound ligand for Eph receptor, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. |
| Description | A DNA sequence encoding the human EFNA4 (NP_005218.1)(Met1-Gly171) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 26 |
| AA Sequence | Met1-Gly171 |
| Molecular Weight | The recombinant human EFNA4/Fc comprises 387 a.a. and has a predicted molecular mass of 43.4 kDa. The apparent molecular mass of the protein is approximately 48.1 and 34.9 kDa in SDS-PAGE under reducing conditions. |
| Purity | (82.7+11.1)% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. Measured by its binding ability in a functional ELISA.2. Immobilized human EPHA7-His at 10 ug/mL(100 uL/well) can bind human EFNA4-Fc (NEW). The EC50 of EFNA4-Fc (NEW) is 2.6-6.1 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
