Recombinant Human Ephrin A3 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1855
Recombinant Human Ephrin A3 Protein (Fc Tag)

Recombinant Human Ephrin A3 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1855
Catalog No.: BLPSN-1855

Product Overview

Tag Fc
Host Species Human
Accession NP_004943.1
Synonym EFL2, Ehk1-L, EPLG3, LERK3
Background Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.
Description A DNA sequence encoding the human EphrinA3 (NP_004943.1) (Met 1-Ser 213) with the C-terminal propeptide removed was fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Gln 23
AA Sequence Met 1-Ser 213
Molecular Weight The recombinant human EphrinA3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 429 a.a. and has a predicted molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3/Fc monomer is approximately 60-65 kDa due to glycosylation.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its binding ability in a functional ELISA. Immobilized mouse EphA6 at 1 ug/ml (100 ul/well) can bind human EphrinA3 / Fc Chimera. The EC50 of human EphrinA3 is 299.2 ng/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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