Recombinant Human EphB4 Protein

Beta LifeScience SKU/CAT #: BLPSN-1839
Recombinant Human EphB4 Protein

Recombinant Human EphB4 Protein

Beta LifeScience SKU/CAT #: BLPSN-1839
Catalog No.: BLPSN-1839

Product Overview

Tag N/A
Host Species Human
Accession NP_004435.3
Synonym HTK, MYK1, TYRO11
Background Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Description The mature form of human EphB4 (NP_004435.3) extracellular domain (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Source HEK293
Predicted N Terminal Leu 16
AA Sequence Met 1-Ala 539
Molecular Weight The recombinant human EphB4 is a monomeric protein consisting of 530 a.a. and has a calculated molecular mass of 57.7 kDa. As a result of glycosylation, rhEphB4 migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
Formulation Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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