Recombinant Human EphB3 Protein (aa 585-998, His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1836
Recombinant Human EphB3 Protein (aa 585-998, His & GST Tag)

Recombinant Human EphB3 Protein (aa 585-998, His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1836
Catalog No.: BLPSN-1836

Product Overview

Tag His&GST
Host Species Human
Accession P54753
Synonym ETK2, HEK2, TYRO6
Background Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Description A DNA sequence encoding the human EPHB3 (P54753) (Gln585-Val998) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Gln585-Val998
Molecular Weight The recombinant human EPHB3 /GST chimera consists of 651 a.a. and has a calculated molecular mass of 74.7 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The specific activity was determined to be 90 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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