Recombinant Human EphB2 Protein (aa 570-987, His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1831
Recombinant Human EphB2 Protein (aa 570-987, His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1831
Catalog No.: BLPSN-1831
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | P29323 |
| Synonym | CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5 |
| Background | Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. |
| Description | A DNA sequence encoding the human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Gly570-Val987 |
| Molecular Weight | The recombinant human EPHB2 /GST chimera consists of 655 a.a. and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 3mM DTT, 10% gly. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
