Recombinant Human EphA7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1819
Recombinant Human EphA7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1819
Catalog No.: BLPSN-1819
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_004431.1 |
| Synonym | EHK-3, EHK3, EK11, EPHA7, HEK11 |
| Background | Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas. |
| Description | A DNA sequence encoding the human EPHA7 (NP_004431.1) extracellular domain (Met 1-Ile 556) was fused with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 28 |
| AA Sequence | Met 1-Ile 556 |
| Molecular Weight | The recombinant human EPHA7 consists of 540 a.a. and has a predicted molecular mass of 60.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EPHA7 is approximately 70-80 kDa due to glycosylation. |
| Purity | >98% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human EphA7 at 2 ug/ml (100 ul/well) can bind mouse EphrinA4 with a linear range of 0.08-10 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
