Recombinant Human ENSA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1766
Recombinant Human ENSA Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1766
Catalog No.: BLPSN-1766
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | O43768 |
| Synonym | ARPP-19e |
| Background | Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents. |
| Description | A DNA sequence encoding the mature form of human ENSA (O43768-1) (Met1-Glu121) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Met1-Glu121 |
| Molecular Weight | The recombinant human ENSA consists of 136 a.a. and predicts a molecular mass of 15.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
