Recombinant Human ENPP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1760
Recombinant Human ENPP2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1760
Catalog No.: BLPSN-1760
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | AAH34961.1 |
| Synonym | ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2 |
| Background | ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection. |
| Description | A DNA sequence encoding the mature form of human ENPP2 (AAH34961.1) (Asp 49-Ile 863) was expressed, with a His tag at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | His |
| AA Sequence | Asp 49-Ile 863 |
| Molecular Weight | The recombinant human ENPP2 consists of 834 a.a. and has a calculated molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhENPP2 is approximately 120-130 kDa due to glycosylation. |
| Purity | >88% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
