Recombinant Human EGFR Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1733
Recombinant Human EGFR Protein (His Tag)

Recombinant Human EGFR Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1733
Catalog No.: BLPSN-1733

Product Overview

Tag His
Host Species Human
Accession NP_005219
Synonym ERBB, ERBB1, HER1, mENA, NISBD2, PIG61
Background As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal His tag.
Source HEK293
Predicted N Terminal Leu 25
AA Sequence Met 1-Ser 645
Molecular Weight The recombinant human EGF receptor consists of 630 a.a. and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to bind human EGF in a functional ELISA.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed