Recombinant Human EGFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1732
Recombinant Human EGFR Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1732
Catalog No.: BLPSN-1732
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_005219 |
| Synonym | ERBB, ERBB1, HER1, mENA, NISBD2, PIG61 |
| Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the extracellular domain (Met 1-Lys 642) of human EGFR (NP_005219) was expressed with a C-terminal His tag. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Leu 25 |
| AA Sequence | Met 1-Lys 642 |
| Molecular Weight | The recombinant human EGFR consists of 626 a.a. and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 ug/ml can bind human EGFR with a linear range of 3.2-400 ng/ml. |
| Formulation | Lyophilized from sterile 20mM Tris, 50mM NaCl, 3mM DTT, 0.5mM TCEP, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
