Recombinant Human EEF1B2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1705

Recombinant Human EEF1B2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1705
Catalog No.: BLPSN-1705
Product Overview
Tag | His |
Host Species | Human |
Accession | P24534 |
Synonym | EEF1B, EEF1B1, EF1B |
Background | EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again. |
Description | A DNA sequence encoding the human EEF1B2 (P24534)(Met1-Ile225) was expressed with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | His |
AA Sequence | Met1-Ile225 |
Molecular Weight | The recombinant human EEF1B2 consists of 240 a.a. and predicts a molecular mass of 26.6 KDa. It migrates as an approximately 30 KDa band in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, 1mM DTT, pH 8.0.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |